– March 10, 2017
My paper from my postdoctoral work at the University of Wisconsin-Madison has been just published in Insects, in special issue “Invasive insect species:
Avanesyan, A., Jaffe, B.D., and C. Guédot (2017) Isolating spermatheca and determining mating status of the invasive spotted wing drosophila, Drosophila suzukii: a protocol for tissue dissection and its applications. Insects: Special issue “Invasive Insect Species”. 8(1), 32; doi:10.3390/insects8010032. Invited paper.
This paper describes a step-by-step protocol for tissue dissection, isolating spermathecae, and determining the mating status of females which I developed specifically for invasive Drosophila suzukii.
Many thanks to Claire Mattmiller, a high school student whom I mentored, for numerous fly dissections she made and several illustrations which have been included in this manuscript.